For an up-to-date list of Jackson Lab publications – click here to view Colin Jackson’s Google Scholar Publication Page. 



Shah, M. V., Antoney, J., Kang, S. W., Warden, A. C., Hartley, C. J., Nazem-Bokaee, H., Jackson, C. J. & Scott, C. Cofactor Under-Explored F420-Dependent Resource Enzymes: for Asymmetric An Redox Biocatalysis. Novel Enzyme and Whole-Cell Biocatalysts 119 (2020). 

Henneberger, C., Bard, L., Panatier, A., Reynolds, J. P., Kopach, O., Medvedev, N. I., Minge, D., Herde, M. K., Anders, S., Kraev, I., Heller, J. P., Rama, S., Zheng, K., Jensen, T. P., Sanchez-Romero, I., Jackson, C. J., Janovjak, H., Ottersen, O. P., Nagelhus, E. A., Oliet, S. H. R., Stewart, M. G., Nägerl, U. V. & Rusakov, D. A. LTP Induction Boosts Glutamate Spillover by Driving Withdrawal of Perisynaptic Astroglia. Neuron (2020). doi:10.1016/j.neuron.2020.08.030

Herde, M. K., Bohmbach, K., Domingos, C., Vana, N., Komorowska-Müller, J. A., Passlick, S., Schwarz, I., Jackson, C. J., Dietrich, D., Schwarz, M. K. & Henneberger, C. Local Efficacy of Glutamate Uptake Decreases with Synapse Size. Cell Rep. 32, 108182 (2020).

Hendler, A., Akiva, E., Sandhu, M., Goldberg, D., Arbely, E., Jackson, C. J. & Aharoni, A. Human SIRT1 Multispecificity Is Modulated by Active-Site Vicinity Substitutions during Natural Evolution. Mol. Biol. Evol. (2020). doi:10.1093/molbev/msaa244

Grinter, R., Ney, B., Brammananth, R., Barlow, C. K., Cordero, P. R. F., Gillett, D. L., Izoré, T., Cryle, M. J., Harold, L. K., Cook, G. M., Taiaroa, G., Williamson, D. A., Warden, A. C., Oakeshott, J. G., Taylor, M. C., Crellin, P. K., Jackson, C. J., Schittenhelm, R. B., Coppel, R. L. & Greening, C. Cellular and Structural Basis of Synthesis of the Unique Intermediate Dehydro-F420-0 in Mycobacteria. mSystems 5, (2020).

Li, D., Damry, A. M., Petrie, J. R., Vanhercke, T., Singh, S. P. & Jackson, C. J. Consensus Mutagenesis and Ancestral Reconstruction Provide Insight into the Substrate Specificity and Evolution of the Front-End Δ6-Desaturase Family. Biochemistry 59, 1398–1409 (2020).

Rapson, T. D., Christley-Balcomb, A. M., Jackson, C. J. & Sutherland, T. D. Enhancement of metallomacrocycle-based oxygen reduction catalysis through immobilization in a tunable silk-protein scaffold. J. Inorg. Biochem. 204, 110960 (2020).

Rapson, T. D., Ju, H., Marshall, P., Devilla, R., Jackson, C. J., Giddey, S. & Sutherland, T. D. Engineering a solid-state metalloprotein hydrogen evolution catalyst. Sci. Rep. 10, 3774 (2020).

Lee, B. M., Harold, L. K., Almeida, D. V., Afriat-Jurnou, L., Aung, H. L., Forde, B. M., Hards, K., Pidot, S. J., Ahmed, F. H., Mohamed, A. E., Taylor, M. C., West, N. P., Stinear, T. P., Greening, C., Beatson, S. A., Nuermberger, E. L., Cook, G. M. & Jackson, C. J. Predicting nitroimidazole antibiotic resistance mutations in Mycobacterium tuberculosis with protein engineering. PLoS Pathog. 16, e1008287 (2020).

Chandler, P. G., Tan, L. L., Porebski, B. T., Green, J. S. & Riley, B. T. Mutational and biophysical robustness in a pre-stabilized monobody. bioRxiv (2020). at <;

Mater, A. C., Sandhu, M. & Jackson, C. The NK Landscape as a Versatile Benchmark for Machine Learning Driven Protein Engineering. Cold Spring Harbor Laboratory 2020.09.30.319780 (2020). doi:10.1101/2020.09.30.319780

Spence, M. A., Mortimer, M., Buckle, A. M. & Jackson, C. J. A comprehensive phylogenetic analysis of the serpin superfamily. Cold Spring Harbor Laboratory 2020.09.09.289108 (2020). doi:10.1101/2020.09.09.289108

Vongsouthi, V., Whitfield, J. H., Unichenko, P., Mitchell, J. A., Breithausen, B., Khersonsky, O., Kremers, L., Monai, H., Hirase, H., Fleishman, S. J., Henneberger, C. & Jackson, C. J. A computationally designed fluorescent biosensor for D-serine. Cold Spring Harbor Laboratory 2020.08.18.255380 (2020). doi:10.1101/2020.08.18.255380

Kaczmarski, J. A., Mahawaththa, M. C., Feintuch, A., Clifton, B. E., Adams, L. A., Goldfarb, D., Otting, G. & Jackson, C. J. Altered conformational sampling along an evolutionary trajectory changes the catalytic activity of an enzyme. Nat. Commun. 11, 5945 (2020).

Lewis, F. J., Chatterjee, D., Kaczmarski, J., Gao, X. & Cai, Y. Avid binding by B cells to the Plasmodium circumsporozoite protein repeat suppresses responses to protective subdominant epitopes. bioRxiv (2020). at <;

Esquirol, L., Peat, T. S., Sugrue, E., Balotra, S., Rottet, S., Warden, A. C., Wilding, M., Hartley, C. J., Jackson, C. J., Newman, J. & Scott, C. Bacterial catabolism of s-triazine herbicides: biochemistry, evolution and application. Adv. Microb. Physiol. 76, 129–186 (2020).

Chandler, P. G., Broendum, S. S., Riley, B. T., Spence, M. A., Jackson, C. J., McGowan, S. & Buckle, A. M. Strategies for Increasing Protein Stability. Methods Mol. Biol. 2073, 163–181 (2020).


Kaczmarski, J. A., Hong, N.-S., Mukherjee, B., Wey, L. T., Rourke, L., Förster, B., Peat, T. S., Price, G. D. & Jackson, C. J. Structural Basis for the Allosteric Regulation of the SbtA Bicarbonate Transporter by the PII-like Protein, SbtB, from Cyanobium sp. PCC7001. Biochemistry 58, 5030–5039 (2019).

Bolte, B., Bryan, C. S., Sharp, P. P., Sayyahi, S., Rihouey, C., Kendrick, A., Lan, P., Banwell, M. G., Jackson, C. J., Fraser, N. J. & Others. Total Syntheses of the 3 H-Pyrrolo [2, 3-c] quinolone-Containing Alkaloids Marinoquinolines A–F, K, and Aplidiopsamine A Using a Palladium-Catalyzed Ullmann Cross-Coupling/Reductive Cyclization Pathway. J. Org. Chem. 85, 650–663 (2019).

Yang, G., Anderson, D. W., Baier, F., Dohmen, E., Hong, N., Carr, P. D., Kamerlin, S. C. L., Jackson, C. J., Bornberg-Bauer, E. & Tokuriki, N. Higher-order epistasis shapes the fitness landscape of a xenobiotic-degrading enzyme. Nat. Chem. Biol. 15, 1120–1128 (2019).

Williams, S. M., An, J. Y., Edson, J., Watts, M., Murigneux, V., Whitehouse, A. J. O., Jackson, C. J., Bellgrove, M. A., Cristino, A. S. & Claudianos, C. An integrative analysis of non-coding regulatory DNA variations associated with autism spectrum disorder. Mol. Psychiatry 24, 1707–1719 (2019).

Correy, G. J., Zaidman, D., Harmelin, A., Carvalho, S., Mabbitt, P. D., Calaora, V., James, P. J., Kotze, A. C., Jackson, C. J. & London, N. Overcoming insecticide resistance through computational inhibitor design. Proc. Natl. Acad. Sci. U. S. A. 116, 21012–21021 (2019).

Zhang, W. H., Carter, B. M., Day, G. J., Govan, N., Jackson, C. & Perriman, A. W. Sequential Electrostatic Assembly of a Polymer Surfactant Corona Increases Activity of the Phosphotriesterase arPTE. Bioconjug. Chem. 30, 2771–2776 (2019).

Shah, M. V., Antoney, J., Kang, S. W., Warden, A. C., Hartley, C. J., Nazem-Bokaee, H., Jackson, C. J. & Scott, C. Cofactor F420-Dependent Enzymes: An Under-Explored Resource for Asymmetric Redox Biocatalysis. Catalysts 9, 868 (2019).

Zammit, N. W., Siggs, O. M., Gray, P. E., Horikawa, K., Langley, D. B., Walters, S. N., Daley, S. R., Loetsch, C., Warren, J., Yap, J. Y., Cultrone, D., Russell, A., Malle, E. K., Villanueva, J. E., Cowley, M. J., Gayevskiy, V., Dinger, M. E., Brink, R., Zahra, D., Chaudhri, G., Karupiah, G., Whittle, B., Roots, C., Bertram, E., Yamada, M., Jeelall, Y., Enders, A., Clifton, B. E., Mabbitt, P. D., Jackson, C. J., Watson, S. R., Jenne, C. N., Lanier, L. L., Wiltshire, T., Spitzer, M. H., Nolan, G. P., Schmitz, F., Aderem, A., Porebski, B. T., Buckle, A. M., Abbott, D. W., Ziegler, J. B., Craig, M. E., Benitez-Aguirre, P., Teo, J., Tangye, S. G., King, C., Wong, M., Cox, M. P., Phung, W., Tang, J., Sandoval, W., Wertz, I. E., Christ, D., Goodnow, C. C. & Grey, S. T. Denisovan, modern human and mouse TNFAIP3 alleles tune A20 phosphorylation and immunity. Nat. Immunol. 20, 1299–1310 (2019).

Kaczmarski, J. A., Mitchell, J. A., Spence, M. A., Vongsouthi, V. & Jackson, C. J. Structural and evolutionary approaches to the design and optimization of fluorescence-based small molecule biosensors. Current Opinion in Structural Biology 57, 31–38 (2019).

Greule, A., Izoré, T., Iftime, D., Tailhades, J., Schoppet, M., Zhao, Y., Peschke, M., Ahmed, I., Kulik, A., Adamek, M., Goode, R. J. A., Schittenhelm, R. B., Kaczmarski, J. A., Jackson, C. J., Ziemert, N., Krenske, E. H., De Voss, J. J., Stegmann, E. & Cryle, M. J. Kistamicin biosynthesis reveals the biosynthetic requirements for production of highly crosslinked glycopeptide antibiotics. Nat. Commun. 10, 2613 (2019).

Wilding, M., Hong, N., Spence, M., Buckle, A. M. & Jackson, C. J. Protein engineering: the potential of remote mutations. Biochem. Soc. Trans. 47, 701–711 (2019).

Bashiri, G., Antoney, J., Jirgis, E. N. M., Shah, M. V., Ney, B., Copp, J., … Jackson, C. J. (2019). A revised biosynthetic pathway for the cofactor F420 in prokaryotes. Nature Communications, 10(1), 1558.

JA Kaczmarski, JA Mitchell, MA Spence, V Vongsouthi, CJ Jackson. (2019) Structural and evolutionary approaches to the design and optimization of fluorescence-based small molecule biosensors. Current opinion in structural biology 57, 31-38

Florian Baier, Nansook Hong, Gloria Yang, Anna Pabis, Charlotte M Miton, Alexandre Barrozo, Paul D Carr, Shina CL Kamerlin, Colin J Jackson, Nobuhiko Tokuriki (2019) Cryptic genetic variation shapes the adaptive evolutionary potential of enzymes. eLife 8, e40789

David C Thorn, Aidan B Grosas, Peter D Mabbitt, Nicholas J Ray, Colin J Jackson, John A Carver (2019) The Structure and Stability of the Disulfide-Linked γS-Crystallin Dimer Provide Insight into Oxidation Products Associated with Lens Cataract Formation. Journal of molecular biology 431 (3), 483-497

T Izoré, J Tailhades, MH Hansen, JA Kaczmarski, CJ Jackson, MJ Cryle (2019). Drosophila melanogaster nonribosomal peptide synthetase Ebony encodes an atypical condensation domain. Proceedings of the National Academy of Sciences, 201811194

Hopkins, D. H., Rane, R. V., Younus, F., Coppin, C. W., Pandey, G., Jackson, C. J., & Oakeshott, J. G. (2019). The molecular basis for the neofunctionalization of the juvenile hormone esterase duplication in Drosophila. Insect Biochemistry and Molecular Biology.


Harold, L. K., Antoney, J., Ahmed, F. H., Hards, K., Carr, P. D., Rapson, T., … Cook, G. M. (2018). FAD-sequestering proteins protect mycobacteria against hypoxic and oxidative stress. The Journal of Biological Chemistry.

Hong, N.-S., Petrović, D., Lee, R., Gryn’ova, G., Purg, M., Saunders, J., Bauer, P., Carr, P. D., Lin, C.-Y., Mabbitt, P. D., Zhang, W., Altamore, T., Easton, C., Coote, M. L., Kamerlin, S. C. L. and Jackson, C. J. (2018) ‘The evolution of multiple active site configurations in a designed enzyme’, Nature Communications, 9(1), p. 3900. doi: 10.1038/s41467-018-06305-y.

94. Zhang, W. H., Herde, M. K., Mitchell, J. A., Whitfield, J. H., Wulff, A. B., Vongsouthi, V., Sanchez-Romero, I., Gulakova, P. E., Minge, D., Breithausen, B., Schoch, S., Janovjak, H., Jackson, C. J. and Henneberger, C. (2018) ‘Monitoring hippocampal glycine with the computationally designed optical sensor GlyFS’, Nature Chemical Biology. doi: 10.1038/s41589-018-0108-2.

93. Kirk, J., Huber, T., & Jackson, C. J. (2018). Chapter 6: Modulating Enzyme Activity via Incorporation of Non-canonical Amino Acids. In G. Williams & M. Hall (Eds.), Modern Biocatalysis: Advances Towards Synthetic Biological Systems (pp. 153–177). The Royal Society of Chemistry.

92. Campbell, E. C., Grant, J., Wang, Y., Sandhu, M., Williams, R. J., Nisbet, D. R., Perriman, A. W., Lupton, D. W. and Jackson, C. J. (2018) ‘Hydrogel-Immobilized Supercharged Proteins’, Advanced Biosystems. Wiley-Blackwell, p. 1700240. doi: 10.1002/adbi.201700240.

91. BE Clifton, JA Kaczmarski, PD Carr, ML Gerth, N Tokuriki, CJ Jackson. (2018). Evolution of cyclohexadienyl dehydratase from an ancestral solute-binding protein.Nature Chemical Biology. 

90. Campbell, E., Correy, G., Mabbitt, P., Buckle, A., Tokuriki, N., Jackson, C. (2018). Laboratory evolution of protein conformational dynamics. Current Opinion in Structural Biology, 50, 49–57. DOI: 10.1016/



89. Ney, B., Carere, C., Sparling, R., Jirapanjawat, T., Stott, M., Jackson, C., … Greening, C. (2017). Cofactor tail length modulates catalysis of bacterial F420-dependent oxidoreductases. Frontiers in Microbiology, 8, 1902.

88.  Hopkins, D. H., Fraser, N. J., Mabbitt, P. D., Carr, P. D., Oakeshott, J. G., & Jackson, C. J. (2017). Structure of an insecticide sequestering carboxylesterase from the disease vector Culex quinquefasciatus: what makes an enzyme a good insecticide sponge? Biochemistry, acs.biochem.7b00774.

87. Correy, G., Zaidman, D., Carvalho, S., Mabbitt, P. D., James, P. J., Kotze, A. C., … Jackson, C. J. (2017). Overcoming insecticide resistance through computational inhibitor design. bioRxiv (Pre-print). Retrieved from

86. Buckler, J. N., Taher, E. S., Fraser, N. J., Willis, C., Carr, P. D., Jackson, C. J., … Banwell, M. G. (2017). The Synthesis of Certain Derivatives and Analogues of (–) – and ( + ) -Galanthamine and an Assessment of their Capacities to Inhibit Acetylcholine Esterase an Assessment of their Capacities to Inhibit Acetylcholine Esterase.

85. Banwell, M., Xu, X., Kim, H.-S., Chen, W.-M., Ma, X., Correy, G. J., … Carr, P. D. (2017). Total Syntheses of the Amaryllidaceae Alkaloids Zephycandidine III and Lycosinine A and Their Evaluation as Inhibitors of Acetylcholinesterase. European Journal of Organic Chemistry.

84. Clifton, B. E., Kaczmarski, J. A., Carr, P. D., Gerth, M. L., Tokuriki, N., Jackson, C. J. (2017) Evolution of an enzyme from a solute-binding protein. Preprint available on bioRxiv:

83. Greening, C., Jirapanjawat, T., Afroze, S., Ney, B., Scott, C., Pandey, G., Lee, B. M., Russell, R. J., Jackson, C. J., Oakeshott, J. G., Taylor, M. C., Warden, A. C. (2017) Mycobacterial F420H2-dependent reductases promiscuously reduce diverse compounds through a common mechanism. Frontiers in Microbiology. doi: 10.3389/fmicb.2017.01000

82. Rouet, R., Langley, D. B., Schofield, P., Christie, M., Roome, B., Porebski, B. T., … Christ, D. (2017). Structural reconstruction of protein ancestry. Proceedings of the National Academy of Sciences, 114(15), 3897–3902.

81. Sugrue, E., Hartley, C. J., Scott, C., & Jackson, C. J. (2016). The evolution of new catalytic mechanisms for xenobiotic hydrolysis in bacterial metalloenzymes. Australian Journal of Chemistry, 69(12), 1383–1395.

80. Faisal Younus, Nicholas J. Fraser, Chris W. Coppin, Jian-Wei Liu, Galen J. Correy, Thomas Chertemps, Gunjan Pandey, Martine Maïbèche, Colin J. Jackson, J. G. O. (2017). Molecular basis for the behavioral effects of the odorant degrading enzyme Esterase 6 in. Scientific Reports, (April), 1–12.

79. Fisher, C. R., Kaczmarski, J. A., Sutton, H. J., Clifton, B., Cai, Y., Dups, J. N., … Cockburn, I. A. (2017). Multivalency drives the neutralizing activity of antibodies against the Plasmodium falciparum circumsporozoite protein. bioRxiv (Pre-Print).

78. Clifton, B. E., Whitfield, J. H., Sanchez-Romero, I., Herde, M. K., Henneberger, C., Janovjak, H., & Jackson, C. J. (2017). Ancestral Protein Reconstruction and Circular Permutation for Improving the Stability and Dynamic Range of FRET Sensors. In V. Stein (Ed.), Synthetic Protein Switches: Methods and Protocols (pp. 71–87). New York, NY: Springer New York.

77. Mitchell, J. A., Zhang, W. H., Herde, M. K., Henneberger, C., Janovjak, H., O’Mara, M. L., & Jackson, C. J. (2017). Method for Developing Optical Sensors Using a Synthetic Dye-Fluorescent Protein FRET Pair and Computational Modeling and Assessment. In V. Stein (Ed.), Synthetic Protein Switches: Methods and Protocols (pp. 89–99). New York, NY: Springer New York.

76. Sugrue, E., Scott, C., & Jackson, C. J. (2017). Constrained evolution of a bispecific enzyme: lessons for biocatalyst design. Org. Biomol. Chem., 15, 937–946.



75. Mohamed, A.E., Condic-Jurkic, K., Ahmed, F.H., Yuan, P., O’Mara, M.L., Jackson, C.J., Coote, M.L. (2016) Hydrophobic shielding drives catalysis of hydride transfer in a family of F420H2-dependent enzymes. Biochemistry. doi: 10.1021/acs.biochem.6b00683.

74. Mitchell, J.A.*, Whitfield, J.H.*, Zhang, W.H.*, Henneberger, C., Janovjak, H., O’Mara, M.L., Jackson, C.J. (2016) Rangefinder: a semisynthetic FRET sensor design algorithm. ACS Sens. doi: 10.1021/acssensors.6b00576. *contributed equally.

73. Sugrue, E.S, Carr, P.D., Scott, C., Jackson, C.J. (2016) Active site desolvation and thermostability tradeoffs in the evolution of catalytically diverse triazine hydrolases. Biochemistry. doi: 10.1021/acs.biochem.6b00731.

72. Li, D., Moorman, R., Vanhercke, T., Petrie, J., Singh, S., Jackson, C.J. (2016) Classification and substrate head-group specificity of membrane fatty acid desaturases. Comput. Struct. Biotechnol. J. 14, 341-349. doi: 10.1016/j.csbj.2016.08.003.

71. Jirapanjawat, T., Ney, B., Taylor, M.C., Warden, A.C., Afroze, S., Russell, R.J., Lee, B.M., Jackson, C.J., Oakeshott, J.G., Pandey, G., Greening, C. (2016) The redox cofactor F420 protects mycobacteria from diverse antimicrobial compounds and mediates a reductive detoxification system. Appl. Environ. Microbiol.  doi: 10.1128/AEM.02500-16.

70. Campbell, E.C., Kaltenbach, M., Correy, G.J., Carr, P.D., Porebski, B.T., Livingstone, E.K., Afriat-Jurnou, L., Buckle, A.M., Weik, M., Hollfelder, F., Tokuriki, N., Jackson, C.J. (2016) The role of protein dynamics in the evolution of new enzyme function. Nat. Chem. Biol. 12, 944-950. doi: 10.1038/nchembio.2175.

69. Sugure, E.S., Hartley, C.J., Scott, C., Jackson, C.J. (2016) The evolution of new catalytic mechanisms for xenobiotic hydrolysis in bacterial metalloenzymes. Aust. J. Chem. . doi: 10.1071/CH16426.

68. Ney, B.*, Ahmed, F.H.*, Carere, C.R., Biswas, A., Warden, A.C., Morales, S.E., Pandey, G., Watt, S.J., Oakeshott, J.G., Taylor, M.C., Stott, M.B., Jackson, C.J., Greening, C. (2016) The methanogenic redox cofactor F420 is widely synthesized by aerobic soil bacteria. ISME J. doi: 10.1038/ismej.2016.100. *contributed equally.

67. Yang, G.*, Hong, N.*, Baier, F., Jackson, C.J., Tokuriki, N. (2016) Conformational tinkering drives evolution of a promiscuous activity through indirect mutational effects. Biochemistry. doi: 10.1021/acs.biochem.6b00561.*contributed equally.

66. Chan, K.X., Mabbitt, P.D., Phua, S.Y., Mueller, J.W., Nisar, N., Gigolashvili, T., Stroeher, E., Grassl, J., Arlt, W., Estavillo, G.M., Jackson, C.J., Pogson, B.J. (2016) Sensing and signaling of oxidative stress in chloroplasts by inactivation of the SAL1 phosphoadenosine phosphatase. Proc. Natl. Acad. Sci. doi: 10.1073/pnas.1604936113.

65. Ahmed, F.H., Mohamed, A.E., Carr, P.D., Lee, B.M., Condic-Jurkic, K., O’Mara, M.L., Jackson, C.J. (2016) Rv2074 is a novel F420H2-dependent biliverdin reductase in Mycobacterium tuberculosis. Protein Sci. 25(9), 1692-709. doi: 10.1002/pro.2975.

64. Correy, G.J., Carr, P.D., Meirelles, T., Mabbitt, P.D., Fraser, N.J., Weik, M., Jackson, C.J. (2016) Mapping the accessible conformational landscape of an insect carboxylesterase using kinetic crystallography and conformational ensemble analysis. Structure 24, 977-987. doi: 10.1016/j.str.2016.04.009.

63. Horgan, C.C., Han, Y.-S., Trueman, H., Jackson, C.J., Sutherland, T.D., Rapson, T.D. (2016) Phosphorescent oxygen-sensing and singlet oxygen production by a biosynthetic silk. RSC Adv. 6, 39530-39533. doi: 10.1039/C6RA03731C. (open access)

62. Greening, C.*, Ahmed, F.H.*, Mohamed, A.E., Lee, B., Pandey, G., Warden, A., Scott, C., Oakeshott, J.G., Taylor, M., Jackson, C.J. (2016) F420- and Fo-dependent redox reactions: physiology, biochemistry, and applications. Microbiol. Mol. Biol. Rev. 80, 429-450. doi: 10.1128/MMBR.00070-15. *contributed equally.

61. Fraser, N.J., Liu, J.W., Mabbitt, P.D., Correy, G.J., Coppin, C.W., Lethier, M., Perugini, M.A., Murphy, J.M., Oakeshott, J.G., Weik, M., Jackson, C.J. (2016) Evolution of protein quaternary structure in response to selective pressure for increased thermostability. J. Mol. Biol. 428, 2359-2371. doi: 10.1016/j.jmb.2016.03.014

60. Mabbitt, P.D.*, Correy, G.J.*, Meirelles, T., Fraser, N.J., Coote, M.L., Jackson, C.J. (2016) Conformational disorganization within the active site of a recently evolved organophosphate hydrolase limits its catalytic efficiency. Biochemistry 55, 1408-1417. doi: 10.1021/acs.biochem.5b01322. *contributed equally.

59. Mohamed, A.E., Ahmed, F.H., Arulmozhiraja, S., Lin, C.Y., Taylor, M.C., Krausz, E.R., Jackson, C.J., Coote, M.L. (2016) Protonation state of F420H2 in the prodrug-activating deazaflavin dependent nitroreductase (Ddn) from Mycobacterium tuberculosis. Mol. BioSyst. 12, 1110-1113. doi: 10.1039/C6MB00033A.

58. Clifton, B.E., Jackson, C.J. (2016) Ancestral protein reconstruction yields insights into adaptive evolution of binding specificity in solute-binding proteins, Cell Chem. Biol. 23, 236-245. doi: 10.1016/j.chembiol.2015.12.010.


57. Ahmed, F.H., Carr, P.D., Lee, B.M., Afriat-Jurnou, L., Mohamed, A.E., Hong, N.-S., Flanagan, J., Taylor, M.C., Greening, C., Jackson, C.J. (2015) Sequence-structure-function classification of a catalytically diverse oxidoreductase superfamily in mycobacteria. J. Mol. Biol. 427, 3554-3571. doi: 10.1016/j.jmb.2015.09.021.

56. Riley, B.T., Broendum, S.S., Reboul, C.F., Cowieson, N.P., Costa, M.G.S., Kass, I., Jackson, C.J., Perahia, D., Buckle, A.M., McGowan, S. (2015) Dynamic motion and communication in the streptococcal C1 phage lysin, PlyC. PLoS One 10, e0140219. doi: 10.1371/journal.pone.0140219. (open access)

55. Greening, C., Biswas, A., Carere, C.R., Jackson, C.J., Taylor, M.C., Stott, M.B., Cook, G.M., Morales, S.E. (2015) Genomic and metagenomic surveys of hydrogenase distribution indicate H2 is a widely utilised energy source for microbial growth and survival. ISME J. doi: 10.1038/ismej.2015.153.

54. Kaltenbach, M., Jackson, C.J. Campbell, E.C., Hollfelder, F., Tokuriki, N. (2015) Reverse evolution leads to genotypic incompatibility despite functional and active-site convergence. eLife 4, e06492. doi: 10.7554/eLife.06492. (open access)

53. Whitfield, J.H., Zhang, W.H., Herde, M.K., Clifton, B.E., Radziejewski, J., Janovjak, H., Henneberger, C., Jackson, C.J. (2015) Construction of a robust and sensitive arginine biosensor through ancestral protein reconstruction. Protein Sci. 24, 1412-1422. doi: 10.1002/pro.2721.

52. Van Benschoten, A.H., Afonine, P.V., Terwilliger, T.C., Wall, M.E., Jackson, C.J., Sauter, N.K., Adams, P.D., Urzhumtsev, A., Fraser, J.S. (2015) Predicting X-ray diffuse scattering from translation-libration-screw structural ensembles. Acta Crystallogr. D Biol. Crystallogr. 71, 1657-1667. doi: 10.1107/S1399004715007415

51. Sugrue, E., Fraser, N.J., Hopkins, D.H., Carr, P.D., Khurana, J.L., Oakeshott, J.G., Scott, C., Jackson, C.J. (2015) Evolutionary expansion of the amidohydrolase superfamily in bacteria in response to synthetic compounds: the molinate and diuron hydrolases. Appl. Environ. Microbiol. 71, 2612-2624. doi: 10.1128/AEM.04016-14.


50. Carruthers, T.J., Carr, P.D., Loh, C.T., Jackson, C.J., Otting, G. (2014) Iron(III) located in the dinuclear metallo-β-lactamase IMP-1 by pseudocontact shifts. Angew. Chem. Int. Ed. 53, 14269-14272. doi: 10.1002/anie.201408693.

49. Tokuriki N., Jackson C.J. (2014) Enzyme dynamics and engineering: one step at a time. Chem. Biol.21, 1259-1260. doi: 10.1016/j.chembiol.2014.10.003.

48. Lan P., Jackson C.J., Banwell M.G., Willis A.C. (2014) Synthesis of a D-ring isomer of galanthamine via a radical-based Smiles rearrangement reaction. J. Org. Chem.  79, 6759-64. doi: 10.1021/jo501255c.

47. Carr P.D., Ewens C.L., Dai J., Ollis D.L., Murphy J.M., Jackson C.J., Young I.G. (2014) Crystal structure of the mouse interleukin-3 β-receptor: insights into interleukin‑3 binding and receptor activation.  Biochem J. doi:

46. Jackson C.J., Coppin C.W., Carr P.D., Aleksandrov A., Wilding M., Sugrue E., Ubels J., Paks M., Newman J., Peat T.S., Russell R.J., Field M., Weik M., Oakeshott J.G., Scott C.. (2014) 300-fold increase in production of the Zn2+-dependent dechlorinase TrzN in soluble form via apoenzyme stabilization. Appl. Environ. Microbiol. 80, 4003-11. doi: 10.1128/AEM.00916-14.

45. Naqvi T., Warden A.C., French N., Sugrue E., Carr P.D., Jackson C.J., Scott C. (2014)  A 5000-fold increase in the specificity of a bacterial phosphotriesterase for malathion through combinatorial active site mutagenesis. PLoS One. 9, e94177. doi: 10.1371/journal.pone.0094177.

44. Jackson C.J., Carville A., Ward J., Mansfield K., Ollis D.L., Khurana T., Bird S.B. (2014) Use of OpdA, an organophosphorus (OP) hydrolase, prevents lethality in an African green monkey model of acute OP poisoning. Toxicology 317, 1-5. doi: 10.1016/j.tox.2014.01.003


43. Jackson C.J., Liu J.-W., Carr P.D., Younus F., Coppin C., Meirelles T., Lethier M., Pandey G., Ollis D.L., Russell R.J., Weik M., Oakeshott J.G. Structure and function of an insect α-carboxylesterase (αEsterase7) associated with insecticide resistance. Proc. Nat. Acad. Sci. USA (2013), 110(25), 10177-10182.

42. Zhang W.H., Otting G., Jackson C.J. Protein engineering with unnatural amino acids. Curr. Opin. Struct. Biol. (2013), 23(4), 581-587.


41. Afriat-Jurnou L., Jackson C.J., Tawfik D.S. Reconstructing a missing link in the evolution of a recently diverged phosphotriesterase by active-site loop remodeling. Biochemistry (2012), 51(31), 6047-6055.

40. Coppin C.W., Jackson C.J., Sutherland T., Hart P.J., Devonshire A.L., Russell R.J., Oakeshott J.G. Testing the evolvability of an insect carboxylesterase for the detoxification of synthetic pyrethroid insecticides. Insect Biochem. Mol. Biol. (2012), 42(5), 343-352.

39. Gallat F.-X., Brogan A.P.S., Fichou Y., McGrath N., Moulin M., Hartlein M., Combet J., Wuttke J., Mann S., Zaccai G., Jackson C.J., Perriman A.W., Weik M. A polymer surfactant corona dynamically replaces water in solvent-free protein liquids and ensures macromolecular flexibility and activity. J. Am. Chem. Soc. (2012), 134(32), 13168-13171.

38. Noor S., Taylor M.C., Russell R.J., Jermiin L.S., Jackson C.J., Oakeshott J.G., Scott C. Intramolecular epistasis and the evolution of a new enzymatic function. PLoS One (2012), 7(6), e39822/1-11.

37. Sharma P., Pandey R., Kumari K., Pandey G., Jackson C.J., Russell R.J., Oakeshott J.G., Lal R. Kinetic and sequence-structure-function analysis of known LinA variants with different hexachlorocyclohexane isomers. PLoS One (2011), 6(9), e25128.

36. Tokuriki N., Jackson C.J., Afriat-Jurnou L., Wyganowski K.T., Tang R., Tawfik D.S. Diminishing returns and tradeoffs constrain the laboratory optimization of an enzyme. Nat. Commun. (2012), 3(12), 1257/1-9.


35. Brittain D.R.B., Pandey R., Kumari K., Sharma P., Pandey G., Lal R., Coote M.L., Oakeshott J.G., Jackson C.J. Competing SN2 and E2 reaction pathways for hexachlorocyclohexane degradation in the gas phase, solution and enzymes. Chem. Commun. (2011), 47(3), 976-978.

34. Fraser J.S., Jackson C.J. Mining electron density for functionally relevant protein polysterism in crystal structures. Cell. Mol. Life Sci. (2011), 68(11), 1829-1841.

33. Russell R.J., Scott C., Jackson C.J., Pandey R., Pandey G., Taylor M.C., Coppin C.W., Liu J.-W., Oakeshott J.G. The evolution of new enzyme function: lessons from xenobiotic metabolizing bacteria versus insecticide-resistant insects. Evol. Appl. (2011), 4(2), 225-248.

32. Sharma P., Pandey R., Kumari K., Pandey G., Jackson C.J., Russell R.J., Oakeshott J.G., Lal R Kinetic and sequence-structure-function analysis of known LinA variants with different hexachlorocyclohexane isomers. PLoS One (2011), 6(9), e25128/1-4.

31. Ugwumba I.N., Ozawa K., Xu Z.-Q., Ely F., Foo J.-L., Herlt A.J., Coppin C., Brown S., Taylor M.C., Ollis D.L., Mander L.N., Schenk G., Dixon N.E., Otting G., Oakeshott J.G., Jackson C.J. Improving a natural enzyme activity through incorporation of unnatural amino acids. J. Am. Chem. Soc. (2011), 133(2), 326-333.



30. Foo, J.-L., Jackson C.J., Carr P.D., Kim H.-K., Schenk G., Gahan L.R., Ollis D.L. Mutation of outer-shell residues modulates metal ion co-ordination strength in a metalloenzyme. Biochem. J. (2010), 429(2), 313-321.

29. Gresham C., Rosenbaum C., Gaspari R.J., Jackson C.J., Bird S.B. Kinetics and efficacy of an organophosphorus hydrolase in a rodent model of methyl-parathion poisoning. Acad. Emerg. Med. (2010), 17(7), 736-740.

28. Jackson C.J., Gillam E.M.J., Ollis D.L. Directed evolution of enzymes. In Modern Methods in Natural Products Chemistry, Mander L.N., ed. Comprehensive Natural Products Chemistry II: Chemistry and Biology, Vol. 9, Elsevier Science: Oxford, UK (2010), pp. 723-749.

27. Jackson C.J., Oakeshott J.G., Sanchez-Hernandez J.C., Wheelock, C.E. Caboxylesterases in the metabolism and toxicity of pesticides. In Anticholinesterase Pesticides: Metabolism, Neurotoxicity, and Epidemiology, Satoh T., Gupta R.C., eds. John Wiley and Sons, Hoboken, NJ, USA (2010), pp. 57-75.

26. Jackson C.J., Scott C., Carville A., Mansfield K., Ollis D.L., Bird S.B. Pharmacokinetics of OpdA, an organophosphorus hydrolase, in the African green monkey. Biochem. Pharmacol. (2010), 80(7), 1075-1079.

25. Lal R., Pandey G., Sharma P., Kumari K., Malhotra S., Pandey R., Raina V., Kohler H.-P.E., Holliger C., Jackson C.J., Oakeshott J.G. Biochemistry of microbial degradation of hexachlorocyclohexane and prospects for bioremediation. Microbiol. Mol. Biol. Rev. (2010) 74(1), 58-80.

24. Taylor M.C., Jackson C.J., Tattersall D.B., French N., Peat T.S., Newman J., Briggs L.J., Lapalikar G.V., Campbell P.M., Scott C., Russell R.J., Oakeshott J.G. Identification and characterization of two families of F420H2-dependent reductases from Mycobacteria that catalyze aflatoxin degradation. Mol. Microbiol. (2010), 78(3), 561-575.


23. Khurana J.L., Jackson C.J., Scott C., Pandey G., Russell R.J., Oakeshott J.G. Enzymes and methods for hydrolysing a phenylureas, carbamates and organophosphates. International Patent No. WO2010/003184 A1 (2010), 91 pp.

22. Scott C., Russell R.J., Coppin C.W., Oakeshott J.G. Jackson, C.J. Enzymes and methods for degrading S-triazines and diazines. International Patent No. WO2010/025499 A1 (2010), 91 pp.



21. Jackson C.J., Foo J.-L., Tokuriki N., Afriat L., Carr P.D., Kim H.-K., Schenk G., Tawfik D.S., Ollis D.L. Conformational sampling, catalysis, and evolution of the bacterial phosphotriesterase. Proc. Natl. Acad. Sci. USA (2009), 106(51), 21631-21636.

20. Jackson C.J., Weir K., Herlt A., Khurana J., Sutherland T.D., Horne I., Easton C., Scott C., Russell R.J., Scott C., Oakeshott J.G. Structure-based rational design of a phosphotriesterase. Appl. Environ. Microbiol. (2009), 75(15), 5153-5156.

19. Khersonsky O., Röthlisberger D., Dym O., Albeck S., Jackson C.J., Baker D., Tawfik, D.S. Evolutionary optimization of computationally designed enzymes: Kemp eliminases of the KE07 series. J. Mol. Biol. (2009), 396(4), 1025-1042.

18. Khurana J.L., Jackson C.J., Scott C., Pandey G., Horne I., Russell R.J., Herlt A.J., Easton C.J., Oakeshott J.G. Characterization of the phenylurea hydrolases A and B: founding members of a novel amidohydrolase subgroup. Biochem. J. (2009), 418(2), 431-441.

17. Scott C., Jackson C.J., Coppin C.W., Mourant R.G., Hilton M.E., Sutherland T.D., Russell R.J., Oakeshott J.G. Catalytic improvement and evolution of atrazine chlorohydrolase. Appl. Environ. Microbiol. (2009), 75(7), 2184-2191.

16. Weston D.P., Jackson C.J. Use of engineered enzymes to identify organophosphate and pyrethroid-related toxicity in toxicity identification evaluations. Environ. Sci. Technol. (2009), 43(14), 5514-5520.


15. Scott C., Jackson, C.J., Russell R.J., Oakeshott J.G., Coppin C.W. Enzymes and methods for degrading chlorinated S-triazines. International Patent No. WO2009/076711 A1 (2009), 68 pp.



14. Biswas S., Russell R.J., Jackson C.J., Vidovic M., Ganeshina O., Oakeshott J.G., Claudianos C. Bridging the synaptic gap: neuroligins and neurexin I in Apis mellifera. PLoS One (2008), 3(10), e3452/1-19.

13. Hadler K.S., Tanifum E.A., Yip S.H.-C., Mitic N., Guddat L.W., Jackson C.J., Gahan L.R., Nguyen K., Carr P.D., Ollis D.L., Hengge A.C., Larrabee J.A., Schenk G. Substrate-promoted formation of a catalytically competent binuclear center and regulation of reactivity in a glycerophosphodiesterase fromEnterobacter aerogenes. J. Am. Chem. Soc. (2008), 130(43), 14129-14138.

12. Jackson C.J., Foo J.-L., Kim H.-K., Carr P.D., Liu J.-W., Salem G., Ollis D.L. In crystallo capture of a Michaelis complex and product-binding modes of a bacterial phosphotriesterase. J. Mol. Biol. (2008), 375(5), 1189-1196.

11. Jackson C.J., Hadler K.S., Carr P.D., Oakley A.J., Yip S., Schenk G., Ollis D.L. Malonate-bound structure of the glycerophosphodiesterase from Enterobacter aerogenes (GpdQ) and characterization of the native Fe2+ metal-ion preference. Acta Crystallogr., Sect. F: Struct. Biol. Cryst. Commun. (2008), 64(8), 681-685.

10. Jackson C.J., Taylor M.C., Tattersall D.B., French N.G., Carr P.D., Ollis D.L., Russell R.J., Oakeshott J.G. Cloning, expression, purification, crystallization and preliminary X-ray studies of a pyridoxine 5′-phosphate oxidase from Mycobacterium smegmatis. Acta Crystallogr., Sect. F: Struct. Biol. Cryst. Commun. (2008), 64(5), 435-437.

9. Scott C., Pandey G., Hartley C.J., Jackson, C.J., Cheesman M.J., Taylor M.C., Pandey R., Khurana J.L., Teese M., Coppin C.W., Weir K.M., Jain R.K., Lal R., Russell R.J., Oakeshott J.G. The enzymatic basis for pesticide bioremediation. Indian J. Microbiol. (2008), 48(1), 65-79.



8. Ely F., Foo J.-L., Jackson C.J., Gahan L.R., Ollis D., Schenk G. Enzymatic bioremediation: organophosphate degradation by binuclear metallo-hydrolases. Curr. Top. Biochem. Res. (2007), 9(2), 63-78.

7. Jackson C.J., Carr P.D., Liu J.-W., Watt S.J., Beck J.L., Ollis D.L. The structure and function of a novel glycerophosphodiesterase from Enterobacter aerogenes. J. Mol. Biol. (2007), 367(4), 1047-1062.



6. Jackson C.J., Carr P.D., Kim H.-K., Liu J.-W., Herrald P., Mitic N., Schenk G., Smith C.A., Ollis D.L. Anomalous scattering analysis of Agrobacterium radiobacter phosphotriesterase: the prominent role of iron in the heterobinuclear active site. Biochem. J. (2006), 397(3), 501-508.

5. Jackson C.J., Carr P.D., Kim H.-K., Liu J.-W., Ollis D.L. The purification, crystallization and preliminary diffraction of a glycerophosphodiesterase from Enterobacter aerogenes. Acta Crystallogr., Sect. F: Struct. Biol. Cryst. Commun. (2006), 62(7), 659-661.



4. Jackson C.J, Kim H.-K., Carr P.D., Liu J.-W., Ollis D.L. The structure of an enzyme-product complex reveals the critical role of a terminal hydroxide nucleophile in the bacterial phosphotriesterase mechanism. Biochim. Biophys. Acta (2005), 1752(1), 56-64.

3. Jackson C.J., Liu J.-W., Coote M.L., Ollis D.L. The effects of substrate orientation on the mechanism of a phosphotriesterase. Org. Biomol. Chem. (2005), 3(24), 4343-4350.

2. Yu McLoughlin S., Jackson C.J, Liu J.-W., Ollis D. Increased expression of a bacterial phosphotriesterase in Escherichia coli through directed evolution. Protein Expr. Purif. (2005), 41(2), 433-440.



Yu McLoughlin, S., Jackson, C.J, Liu, J.-W., Ollis, D.L. Growth of Escherichia coli co-expressing phosphotriesterase and glycerophosphodiester phosphodiesterase, using paraoxon as the sole phosphorus source. Appl. Environ. Microbiol. (2004), 70(1), 404-412.

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