New publication

Congratulations to Galen and the E3 team whose paper “Mapping the accessible conformational landscape of an insect carboxylesterase using conformational ensemble analysis and kinetic crystallography” has been published in Structure. Using automated multi-conformer modelling and ensemble refinement, we uncovered a dynamically coupled network important for catalysis in an organophosphate-degrading enzyme, and using kinetic crystallography, weContinue reading “New publication”

New publication

Congratulations to Chris, Hafna, Elaaf and Brendon, whose review “Physiology, biochemistry, and applications of F420- and Fo-dependent redox reactions” was published in Microbiology and Molecular Biology Reviews. The cover highlights some of the proteins that depend on the unique deazaflavin cofactor F420.

New publication

Congratulations to Conor, whose paper “Phosphorescent oxygen-sensing and singlet oxygen production by a biosynthetic silk” was published in RSC Advances. This work shows that porphyrin-based macrocycles can be incorporated into honeybee silk proteins. These functionalised silk films combine the useful properties of the macrocycles, such as their ability to produce antimicrobial singlet oxygen when exposedContinue reading “New publication”

New publication

Congratulations to Nick, Peter, and Galen, whose paper “Evolution of protein quaternary structure in response to selective pressure for increased thermostability” has been published in JMB. This paper shows that when directed evolution was used to impose selective pressure for increased thermostability in an insect carboxylesterase, stabilization was achieved by enrichment of more stable oligomericContinue reading “New publication”

New publication

Congratulations to Peter, Galen and Nick, whose paper “Conformational disorganization within the active site of a recently evolved organophosphate hydrolase limits its catalytic efficiency” was published in Biochemistry. In this paper, we characterised a gain-of-function substitution in an enzyme, which provides resistance to organophosphate pesticides in the Australian sheep blowfly. The full catalytic potential ofContinue reading “New publication”

New publication

Congratulations to Hafna and Elaaf, whose paper “Protonation state of F420H2 in the prodrug-activating deazaflavin dependent nitroreductase (Ddn) from Mycobacterium tuberculosis” was published in Molecular BioSystems. We identified the protonation state of the cofactor of Ddn, which is important for understanding the mechanism by which this enzyme activates anti-mycobacterial prodrugs.

New publication

Congratulations to Ben, whose paper “Ancestral protein reconstruction yields insights into adaptive evolution of binding specificity in solute-binding proteins” has been published in Cell Chemical Biology. This paper gives a new structural and thermodynamic perspective on the evolution of binding specificity and shows the role of conformational plasticity and enthalpic interactions for promiscuous binding inContinue reading “New publication”