Rational protein engineering efforts normally focus on altering parts of proteins that are directly involved in function – e.g. active sites or ligand binding sites. But changes to residues that are remote from these sites can have a large impact on protein structure, dynamics and function. In this review, we discuss recent literature that reports, and rationalises, the successful engineering of proteins at remote sites. As the use of protein technologies expands, exploiting the potential improvements made possible through modifying remote regions will become vital if we are to realise the full potential of protein engineering and design.
Great work Matt Wilding, Nansook and Matt Spence!