New publication

Congratulations to Elena, on the publication of her paper ‘Constrained evolution of a bispecific enzyme: lessons for biocatalyst design’ in Organic and Biomolecular Chemistry. In this work we investigate the impact of intramolecular epistasis on the evolution of atrazine-specific and atrazine/ametryn bispecific triazine hydrolyses (TrzN). Results show that many of the evolutionary trajectories linking the mono-functional and bispecific isoforms are unviable due to inactive intermediates, with the epistatic interactions between mutations being responsible for the bottlenecks in the evolutionary landscape. This work will help guide future work in the design of biocatalysts. 493694C4-05AE-4AEC-ACA9-F9205CC2566F

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s