New publication

Congratulations to Elena, on the publication of her paper ‘Constrained evolution of a bispecific enzyme: lessons for biocatalyst design’ in Organic and Biomolecular Chemistry. In this work we investigate the impact of intramolecular epistasis on the evolution of atrazine-specific and atrazine/ametryn bispecific triazine hydrolyses (TrzN). Results show that many of the evolutionary trajectories linking the mono-functional and bispecific isoforms are unviable due to inactive intermediates, with the epistatic interactions between mutations being responsible for the bottlenecks in the evolutionary landscape. This work will help guide future work in the design of biocatalysts. 493694C4-05AE-4AEC-ACA9-F9205CC2566F

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