New publication

Congratulations to Galen and the E3 team whose paper “Mapping the accessible conformational landscape of an insect carboxylesterase using conformational ensemble analysis and kinetic crystallography” has been published in Structure.

Using automated multi-conformer modelling and ensemble refinement, we uncovered a dynamically coupled network important for catalysis in an organophosphate-degrading enzyme, and using kinetic crystallography, we could monitor the release of a covalently bound intermediate in “slow motion”. This work shows how crystallographic tools can be used to explore the conformational landscape of an enzyme at each step in a complex catalytic cycle.

graphical_abstract

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s